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Abstract

Sweet Regulation of Human Glucocorticoid Receptor Transcriptional Activity

This study is to understanding the transcription profile of human Glucocorticoid Receptor GR, gene of a neuroendocrine, stress response and obesity related receptor. For GR, relative luciferase activity RLA and the distribution of phosphorylation [P], glycosylation sites [G] on transcription factor for promoter model were plotted on the same chart. Within the 3.2 kb upstream of the methionine ATG, for GR, trend lines of RLA with that of [G] or ([P]-[G]), both tend to have negative reciprocal relationship. It brings up the question: for the neuendocrine glucocorticoid receptor, does the nutrition and obesity related glycosylation regulated the transcriptional activity in a negative reciprocal way? In conclusion, for GR, the reciprocal relation between trend line of [G], ([P] ± [G]) or [P] and that of RLA, give a specific digit evidence for the first time to the theory, which the structure related glycosylation and the signal sensing phosphorylation, exhibit either independently or Interactively on regulation of transcription activity. Abbreviations: RLA: Relative Luciferase Activity; Luciferase/β-Galactosidase Activity; DLA: Dual Luciferase Assay; GR: Human Glucocorticoid Receptor; TF: Transcription Factor; P1: Construct Plasmid 1; P7: Construct Plasmid 7. GR-P1: Construct Plasmid 1 for Human Glucocorticoid Receptor; GR-P7: Constructs Plasmid 7 for Human Glucocorticoid Receptor; [P]: Number of Phosphorylation Sites on TF for Promoter Models; [G]: Number of Glycosylation Sites on TF for Promoter Models; [P]-[G]: Number of Difference between Phosphorylation and Glycosylation Sites on TF for Promoter Models; [P]+[G]: Number for Sum of Phosphoylation and Glycosylation Sites on TF for Promoter Models


Author(s):

Xin wen, Wen Wei Zeng



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